ABSTRACT
Scutellaria agrestis A. St. -Hil. ex Benth. , Lamiaceae (trevo-roxo) is cultivated for medicinal purposes by residents of the riverine communities in the State of Amazonas, Brazil. This study aimed to characterize the anatomy and to make histochemical analysis on vegetative organs of S. agrestis. Samples of the leaf, stem and root were collected from five plants cultivated by the communities located in the Solimões river, Amazon. These samples were fixed and prepared following standard techniques for scanning electron microscopy and for light microscopy. Histochemical tests were carried out on sections to detect the main classes of compounds present in the secretion. Numerous glandular trichomes are seen in both leaf and stem of S. agrestis. The leaves are amphi-hypostomatics and show dorsiventral mesophyll. Hydathodes are present at the tip of the marginal teeth. Anthocyanin pigments occur into the epidermal cells of the stem, petiole, and abaxial leaf surface. The petiole is concave-convex shaped and bears collateral vascular bundles. The stem showed square-shaped, evident endoderm, collateral vascular bundles and parenchymatous pith. The root displays a typical protostelic structure. Idioblasts containing mucilage and phenolic compounds occur in the cortex. These data are important, as they can be useful to identify this species, contributing to the quality control of the medicinal plant.
ABSTRACT
The purpose of this paper was to carry out an anatomical and histochemical analysis of the vegetative organs of Cissus verticillata (L.) Nicolson & C.E. Jarvis, Vitaceae, to contribute for the attest the taxonomic identity of the medicinal plant. Samples from root, stem, leaf and tendril were cleared, dissociated and processed according to the usual methodology for observation under light and scanning electron microscopes. Histochemical tests were performed in order to identify polysaccharides, phenolic and lipid compounds. The C. verticillata root is typically protostelic, and the stem is eustelic with collateral bundles. The tendril presents structural organization similar to the stem, suggesting a common origin for both. The petiole has an epidermis with ornamented cuticle; the cortex is composed of collenchyma and parenchyma, and the vascular tissues are arranged in collateral bundles. The leaf blade is amphistomatic with non-glandular and glandular trichomes, and the mesophyll is dorsiventral. The identification of the idioblasts as secretion site of the phenolic compounds, mucilage and terpenoids as being responsible for the potential activity of the plant is of fundamental importance for future bioprospecting research on this species.
ABSTRACT
Anatomical and histochemical investigations of Sphagneticola trilobata (L.) Pruski, Asteraceae, secretory structures in leaves and stems and the seasonal variation of essential oils were carried out. Histochemical techniques enabled the specific location of the essential oil accumulation in the internal (canals) and external structures (trichomes). Histochemical analysis showed that the secretory trichomes produced steroids. The highest yield was obtained from plants collected in winter, when it was registered low temperature and precipitation. The essential oil was characterized by high percentage of hydrocarbon sesquiterpenes, hydrocarbon monoterpenes and low levels of oxygenated sesquiterpenes. The major components were germacrene D (11.9-35.8%), α-phellandrene (1.4-28.5%), α-pinene (7.3-23.8%), E-caryophyllene (4.6-19.0%), bicyclogermacrene (6.0-17.0%), limonene (1.8-15.1%) and α-humulene (4.0-11.6%). The percentage of most of the individual constituents present in S. trilobata essential oil changed significantly during the months.
ABSTRACT
Esse artigo objetivou desenvolver uma reflexão acerca do uso da espiritualidade/religiosidade pelas mães usuárias do Método Canguru numa maternidade conveniada ao Sistema Único de Saúde no Estado do Rio Grande do Norte. A perspectiva metodológica aplicada nesse estudo foi a pesquisa qualitativa, do tipo descritiva. Os dados foram apreendidos a partir da observação participante, das conversas informais, das entrevistas semi-estruturadas e da história de vida das mães, coletados nos encontros do grupo "Mães em Oração", desenvolvido pela equipe de enfermagem nessa maternidade. A análise mostrou que as práticas espirituais e a religiosidade atuam não apenas como suporte nas situações enfrentadas pelas mães na UTIN, mas abre uma possibilidade para o diálogo acerca da espiritualidade no ambiente hospitalar. Essa prática, ainda muito distanciada dos contextos das ações em saúde, contribui na humanização do cuidado, estreitando o vínculo entre mãe, filho, família e equipe multidisciplinar.
This article aimed to develop reflection about the spirituality and religiosity use by mothers, users of Kangaroo Method in a Unified Health System (Sistema Único de Saúde) maternity in Rio Grande do Norte State. The approach used was the qualitative research, using participant observation, informal chats, semi directed interviews, and mothers' life story as procedure to data collection. Data were collected through meetings in Mothers in prayer (Mães em Oração), group developed by nursing team in this maternity. As the result, the analysis showed that the spiritual practice and the religiosity were used as a support in difficult situations at Neonatal Intensive Therapy Unit, as well as allowed the possibility to speak about spirituality in this maternity. This practice, even unconnected with everyday health care, contributes to humanize health care assistance, tightening the bond among mother, child, family and multidisciplinary team as well.
Este trabajo desarrolla una reflexión acerca del uso de la espiritualidad/religiosidad de las madres usuarias del método "Canguru" en una maternidad vinculada al Sistema Único de Salud en el Estado do Rio Grande do Norte. El enfoque metodológico fue la investigación cualitativa a partir de la observación participante, de las conversaciones informales, de las encuestas semi-estructuradas y de la historia de vida de las madres, recogidas en los encuentros del grupo "Mães em Oração". La análisis demostró que las prácticas y la religiosidad actúen no apenas como apoyo en las situaciones enfrentadas por las madres en la UTIN, pero abre en posibilidad para el dialogo a cerca de la espiritualidad en el ambiente hospitalero. Esta práctica, aun distanciada de los contextos de las acciones en salud, contribuye en la humanización de la atención, promoviendo el vínculo entre madre, hijo, familia y equipo multidisciplinatorio.